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TruLine Ammonia Electrode
YSI TruLine Ammonia Electrode Brugsanvisning
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Required Equipment
• Ion meter (preferred) or pH/mV meter.
• Wash bottle with distilled or deionized water.
• . Several clean beakers (glass, 150 mL preferred)
• . Pipette for measuring 10 mL or less
• Graduated cylinder (100 mL)
• * Fill Solution YSI #400367
• ( ) 1000 mg/L ppm Ammonia Standard YSI #400370*
• ) 100 mg/L (ppm Ammonia Standard #400369 YSI
• Ionic Strength Adjustor (ISA) YSI #400368*
• Replacement Membrane Modules (pk/4) YSI #400366**
*Small bottles included with ammonia kit (YSI #400365)ISE
**Two Membrane modules included with ammonia ISE kit.
Overview
The 400365 YSI a - Ammonia Ion Selective Electrode is gas
sensing electrode for measuring ammonia (NH
3
) in aqueous
samples. be used that buffers sample It must with an ISA the
>pH 11 to cause the ammonia in the sample to become
gaseous. Ammonia Electrode behind an The a ISE is pH
ammonia gas permeable – membrane the ammonia gas
causes pH shift that correlated a in the Fill Solution is to
ammonia concentration based on the result of calibrations .
• Technology: Gas sensing technology
• Size and material:
Membrane Body — (ABS) 12 mm OD x 158 L mm
Cap — x (ABS) 16 mm OD 37 L mm
Cable (Coax 00cm – 1type) ; BNC Connector
• a -Reference: The pH electrode inside has single
junction, Ag/AgCl, annular ceramic junction.
• - Features: Replaceable, pre formed membrane module.
The a pH ISA has indicator that indicates the pH is in
range by a (>11) the presence of blue coloration.
• Range: 0.02- 17,000 mg/L
• Reproducibility: ±2%
• @ Slope: 54 to 59 mv/decade 25°C typical
• pH range: >11
• 0 Operating Temperature: to 50°C
• Interferences: Volatile amines
• Applications/Notes: Popular for wastewater,
aquaculture, agriculture, and industrial applications.
Electrode Preparation
1. The glass pH sensor and reference junction of the pH
electrode should be kept conditioned during transit and
storage. the of the solutionUnscrew cap storage bottle
(Soaker and electrode. Bottle) remove the pH
2. Fill a Membrane Module with Fill Solution
a) Add - s 15 20 drop of Fill Solution to the Membrane Module
b) Tap Module ensure bubbles the Membrane to no are
present . in the fill solution
c) Thread Membrane Module the the slowly into Electrode
Cap. If the Module was overfilled, the excess Fill Solution
will leak out. Avoid rupturing the Membrane Module with
too much Fill Solution.
3. Preferably, allow the pH electrode to condition within the
filled 2 Membrane Module for hours prior to use. The
Membrane Module can be conditioned by placing in a low
to mid-range standard for at least 15 minutes prior to use.
Calibration
1. Connect the assembled ammonia electrode to a meter with
BNC input. Meters with an ISE mode can directly display ion
concentration (e.g. display in mg/L). Ensure the meter is
setup for ammonia and be sure to follow the calibration
procedure in the meter user manual.
Alternatively, a meter with a mV mode can be used, although
the instrument will not display the result of calibration (i.e.
calibration curve will need to be created by the user).
2. For highest accuracy, use a meter with automatic
temperature compensation (ATC) and connect a temperature
sensor.
3. Prepare Standards:
a)
At least 2 standards that bracket the expected sample
range must be used. As an example, prepare 1 mg/L and
10 mg/L standards if the expected sample is ~5 mg/L.
Some meters allow up to a 7 point calibration.
b) There must be at least one order of magnitude
difference between high and low standards (e.g. 10
mg/L and 100 mg/L).
c) If standards span more than one order of magnitude
(e.g. 1 mg/L and 100 mg/L), it is best to prepare at least
one mid-range standard (e.g. 10 mg/L).
d) Each standard should have a volume of 100 mL and
should be placed in a 150 mL glass beaker.
e) Standards should be fresh and carefully prepared. It is
best to use a pipette when measuring small volumes
(10 mL or less) of a more concentrated standard.
f) Use the equation C1*V1=C2*V2 when preparing diluted
standards from a more concentrated standard solution.
As an example, if using the YSI 100 mg/L standard as a
stock solution (YSI #400369) to make 100 mL of a 10
mg/L standard: C1=100 mg/L, V1=?, C2=10 mg/L, and
V2=100 mL. V1=(C2*V2)/C1 =10 mL.
Therefore, 10 mL of the 100 mg/L stock standard should
be added to 90 mL of DI water (i.e. solution should be 100
mL) to make a 10 mg/L standard.
Note: It is not recommended to use a very concentrated
standard (e.g. 1000 mg/L) to make a very dilute standard
(e.g. 0.1 mg/L).
4. Place the electrode in the lowest concentration standard and
stir at a constant rate using a stir bar and stir plate, although
the stirring speed should be limited to minimize loss of
ammonia gas. Use the same stirring rate throughout
calibration.
If you do not have a stir plate, it is best to swirl the solution
or use a stir rod once ISA is added.
5. Add 2 mL of ammonia ISA to the first standard:
a) Using a pipette is recommended. The standard solution
should have a pale blue color that persists after ISA is
added.
b) Allow the solution to stir for 1 minute and begin
calibration. Calibration is time sensitive, as ammonia
diffuses from the sample ~4 minutes after ISA is added.
Therefore, ensure calibration with the standard is
complete 1 to 4 minutes after ISA is added. This timing
is critical! If calibration using the standard is
unsuccessful within this time limit, a fresh standard will
n eed to be prepared.
6. After calibrating with the first point, finish calibrating using
steps #4 and #5 for the remaining calibration points.
C alibrate in order of increasing concentration.
7. Evaluate electrode slope:
a) View the calibration record on the ISE meter, or create
a curve if using a meter with only a mV mode.
b) For highest accuracy, the ammonia electrode slope
should be between 52 mV/decade and - -60 mV/decade.
8. Recalibrate often:
a) The ammonia electrode should be calibrated at the
beginning of each day.
b) For best accuracy, it is best to calibrate the ammonia
electrode every 10 samples. If this calibration interval is
too frequent, at least verify your calibration result every
2 hours by preparing a fresh low to mid range standard, -
adding ISA, and verifying the reading. If the mV reading
has changed ~3 mV compared to the reading in that
standard during calibration, you will need to recalibrate
the electrode.
Measurement
Samples must be prepared like standards. Follow steps #3-5
of the calibration procedure when preparing samples and
taking a measurement.
In summary, 100 mL of sample should be collected and 2 mL
of ISA added. The sample should be fresh and the same
stirring rate using during calibration should be utilized. A
measurement can be taken 1 to 4 minutes after ISA is added.
The temperature of measurement solutions should be as
close as possible to solution temperatures during calibration.
Problemløsning YSI TruLine Ammonia Electrode
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Specifikationer
| Mærke: | YSI |
| Kategori: | Ikke kategoriseret |
| Model: | TruLine Ammonia Electrode |